Question: I need help answering these questions shown in the image. The DOG gene is expressed in cats and gives them their fear of large dogs.

I need help answering these questions shown in the image.

The DOG gene is expressed in cats and gives them their fear of large dogs. Studies suggest that the DOG gene is regulated by several proteins, two of which are termed protein 1 and protein 2. These bind to regions in the DOG gene termed region 1 and region 2. A schematic of the DOG gene is shown below. } 2 _ REGULATORY PROTEINS BINDING SITES - TRANSCRIPTION A START SITE FOR THE DOG GENE To determine how these proteins control transcription of the DOG gene, you isolate the gene from various cats and determine the DNA sequences of the binding sites; you also measure the level of DOG mRNA produced in these cats' cells. Interestingly, different cats have different versions of the gene (alleles) that vary in the nucleotide sequence of the binding sites. If the binding site is pormal (wild type) it is indicated by a (+) in the table, and this will mean protein 1 or 2 can bind to its site. If the binding site is mutated it will be indicated with a (-), and this will mean protein 1 or 2 cannot bind to the DNA. Here are your results: SAMPLE SITE 1 SITE 2 LEVEL OF DOG mRNA CAT CATz | - [ %+ [ 5 CAT3 | %+ | - | %0 cAT4 | - | - | @7 CAT 5 Things to consider before you begin the online quiz: What is the function of proteins 1 and 2 in the regulation of the DOG gene? (Are they activators, repressors, or some combination of the two?) * How do Proteins 1 and 2 affect each other's function? What might happen to the regulation of DOG if an additional protein (Protein 3) bound to site 3? How many base-pairs of DNA would be present in the binding sites? In the regions marked A and B? What is the expected status of Site 1 and Site 2 in Cat #5? (+ or -) Would you predict that Cats #2-5 have less fear of dogs? (Why or why not?)

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