import sys

import cv$2$

import numpy as np

from PySide$6.$QtWidgets import $($QApplication$,$QMainWindow$,$ QLabel, QPushButton, QFileDialog$)$

from PySide$6.$QtGui import QPixmap

from PySide$6.$QtUiTools import QUiLoader

from PySide$6.$QtCore import QFile

from matplotlib import pyplot as plt

import matplotlib

matplotlib.use$(\text{'}$QtAgg$\text{'})$

class CellCounterApp$($QMainWindow$)$:

$$ def $\_\_$init$\_\_($self$,$parent$=$None$)$:

$$ super$().\_\_$init$\_\_()$

$$ # Load form.ui file

$$ self.load$\_$ui$()$

$$ # Get references to components

$$ self.image$\_$label $=$self$.$findChild$($QLabel$,"$image$\_$label"$)$

$$ self.result$\_$label $=$self$.$findChild$($QLabel$,"$result$\_$label"$)$

$$ self.load$\_$button $=$self$.$findChild$($QPushButton$,"$load$\_$button"$)$

$$ self.count$\_$button $=$self$.$findChild$($QPushButton$,"$count$\_$button"$)$

$$ # Connect button signals

$$ self.load$\_$button.clicked.connect$($self$.$load$\_$image$)$

$$ self.count$\_$button.clicked.connect$($self$.$count$\_$cells$)$

$$ self.count$\_$button.setEnabled$($False$)$

$$ # Image data attributes

$$ self.image$\_$path $=$None

$$ self.original$\_$image $=$None

$$ def load$\_$ui$($self$)$:

$"""$Loads the form.ui file."""

$$ loader $=$QUiLoader$()$

$$ ui$\_$file $=$QFile$("$form$.$ui$")$ # Path to the form.ui file

$$ ui$\_$file.open$($QFile$.$ReadOnly$)$

$$ self.ui $=$loader$.$load$($ui$\_$file$)$

$$ self.ui$.$setParent$($self$)$

$$ ui$\_$file.close$()$

$$ def load$\_$image$($self$)$:

$"""$Handles loading an image from the file system."""

$$ file$\_$path, $\_=$QFileDialog$.$getOpenFileName$($self$,"$Select Image", $"","$Images $(*.$png $*.$jpg $*.$jpeg $*.$bmp$)")$

$$ if file$\_$path:

$$ self.image$\_$path $=$file$\_$path

$$ self.original$\_$image $=$cv$2.$imread$($self$.$image$\_$path$)$ # Load image with OpenCV

$$ # Show the image on QLabel

$$ pixmap $=$QPixmap$($self$.$image$\_$path$)$

$$ self.image$\_$label.setPixmap$($pixmap$)$

$$ self.result$\_$label.setText$("$Cell Count: N$/$A$")$ # Reset the result label

$$ self.count$\_$button.setEnabled$($True$)$ # Enable the count button

$$ def count$\_$cells$($self$)$:

$"""$Detects and counts cells in the loaded image."""

$$ if self.original$\_$image is None:

$$ return

$$ # Convert the image to grayscale

$$ gray $=$cv$2.$cvtColor$($self$.$original$\_$image, cv$2.$COLOR$\_$BGR$2$GRAY$)$

$$ # Apply Gaussian Blur to reduce noise and smooth the image

$$ blurred $=$cv$2.$GaussianBlur$($gray$,(11,11),0)$

$$ # Apply thresholding to create a binary image

$\_,$binary $=$cv$2.$threshold$($blurred$,127,255,$cv$2.$THRESH$\_$BINARY$\_$INV$)$

$$ # Find contours in the binary image

$$ contours, $\_=$cv$2.$findContours$($binary$,$cv$2.$RETR$\_$EXTERNAL, cv$2.$CHAIN$\_$APPROX$\_$SIMPLE$)$

$$ # Get the number of detected contours

$$ cell$\_$count $=$len$($contours$)$

$$ self.result$\_$label.setText$($f$"$Cell Count: $\{$cell$\_$count$\}")$

$$ # Draw contours on the original image

$$ output$\_$image $=$self$.$original$\_$image.copy$()$

$$ cv$2.$drawContours$($output$\_$image, contours, $-1,(0,255,0),2)$

$$ # Use matplotlib to display the original and processed images side by side

$$ plt$.$figure$($figsize$=(12,6))$

$$ plt$.$subplot$(1,2,1)$

$$ plt$.$title$("$Original Image"$)$

$$ plt$.$imshow$($cv$2.$cvtColor$($self$.$original$\_$image, cv$2.$COLOR$\_$BGR$2$RGB$))$ # Convert BGR to RGB

$$ plt$.$subplot$(1,2,2)$

$$ plt$.$title$("$Contours$")$

$$ plt$.$imshow$($cv$2.$cvtColor$($output$\_$image, cv$2.$COLOR$\_$BGR$2$RGB$))$

$$ from matplotlib.backends.backend$\_$qt$5$agg import FigureCanvasQTAgg as FigureCanvas

$$ # You can display images directly in the QLabel or another Qt widget during the cell counting process.

if $\_\_$name$\_\_=="\_\_$main$\_\_"$:

$"""$Entry point of the application."""

$$ app $=$QApplication$($sys$.$argv$)$

$$ window $=$CellCounterApp$()$

$$ window.show$()$

$$ sys$.$exit$($app$.$exec$())$

when I run this code on QTCreator, it shows this error every time:$2024-12-3103$:$06$:$40\mathrm{.}457$Python$[59090$:$4762284]+[$IMKClient subclass$]$: chose IMKClient$\_$Modern

$2024-12-3103$:$06$:$40\mathrm{.}457$Python$[59090$:$4762284]+[$IMKInputSession subclass$]$: chose IMKInputSession$\_$Modern

QCoreApplication::exec: The event loop is already running