Question: In a recombinant protein expression process, the host microorganism, Pichia pastoris, must be separated from the culture supernatant after production. A pilot scale bioreactor with

In a recombinant protein expression process, the host microorganism, Pichia pastoris, must be separated from the culture supernatant after production. A pilot scale bioreactor with a working volume of 250 liters is used for this process and the final biomass concentration is 330 g/L. Considering the separation, it should be completed in 1 hour.
What kind of chromatography would you use to purify this protein?

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ANSWER The best chromatography method that can be used in purifying this protein is Affinity chromat... View full answer

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