Q2: In a recombinant protein expression process the host microorganism, Pichia pastoris needs to be separated...

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Q2: In a recombinant protein expression process the host microorganism, Pichia pastoris needs to be separated from the culture supernatant after production. A pilot scale bioreactor with a working volume of 250 liters is used for this process and the final biomass concentration is 330 g/L. Considering the separation should be finished in 1 hour: a) Calculate the sigma factor () of a continuous disc stack centrifuge that can fulfil this task assuming density of the microorganism is 1.2 g/L, average size is 3μm (round shape). density of the supernatant is 1.05 g/L. b) If you wanted to reduce the duration of this separation process by 50%, what would you change? Please consider and mention the benefits and disadvantages of such a change. Q2: In a recombinant protein expression process the host microorganism, Pichia pastoris needs to be separated from the culture supernatant after production. A pilot scale bioreactor with a working volume of 250 liters is used for this process and the final biomass concentration is 330 g/L. Considering the separation should be finished in 1 hour: a) Calculate the sigma factor () of a continuous disc stack centrifuge that can fulfil this task assuming density of the microorganism is 1.2 g/L, average size is 3μm (round shape). density of the supernatant is 1.05 g/L. b) If you wanted to reduce the duration of this separation process by 50%, what would you change? Please consider and mention the benefits and disadvantages of such a change.

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a The sigma factor of a continuous disk stack centrifuge that can fulfill this task is 28 The calcul... View the full answer