Problem 3 (35 points) (a) The parent culture for a bacterial fermentation was grown at 30C in a medium containing 60 g1 glucose, 20 31 protein hydrolysate and 2031 yeast extract Samples were inoculated into four subcultures as follows: 2 Subculture A B Medium as for parent as for parent D see below as for parent culture culture 10 culture 10* 10 10 Inoculum size (cell) Inoculum age (h) 6 24 24 24 The medium for subculture D contained: 60g/l glucose, 1091 (NH) SO... 1.01 MgSo.. 1.0 p1 CaCl, and assorted trace elements. The figure shows the growth of the four subcultures: ln (cells ml) Time Give a detailed explanation of the differences in the behaviour of 0 subcultures A and B subcultures B and D subcultures B and C. In your answer make reference to the biochemical and microbiological mechanisms operating in the various growth phases. (9 points, 3 points each)