Question: Problem 335 points (a) The parent culture for a bacterial fermentation was grown at 30Cina medium containing glucose, 20 g protein hydrolyte and 20gyeast extract

Problem 335 points (a) The parent culture for a bacterial fermentation was grown at 30Cina medium containing glucose, 20 g protein hydrolyte and 20gyeast extract Samples were inoculated into four subcultures as follow 2 D see below Medium as for parent culture 107 Subculture B C as for parent as for parent culture culture 10 10+ Inoculum size {cclls ml) Inaculumage 6 24 24 24 th) The medium for subculture contained: 6091 glucose, 103 1(NH..SO 1.01 MgSO4, 1.0 g1 Cach and assorted trace elements The figure shows the growth of the four subcultures In (cellsml) Time Give a detailed explanation of the differences in the behaviour of wubcultures A and B () Muscultures Band D subcultures Band In your answer make reference to the biochemical and microbiological mechanisms operating in the various growth plus points. 3 points each

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