Problem 3 (35 points) (a) The parent culture for a bacterial fermentation was grown at 30C in a medium containing 60 g/l glucose, 20 g/1 protein hydrolysate and 20g/l yeast extract Samples were inoculated into four subcultures as follows: 2 Medium A as for parent culture 106 Subculture B as for parent as for parent culture culture 10% 104 D see below Inoculum size (cells/ml) 10% Inoculum age 6 24 24 24 (h) The medium for subculture D contained: 60g/l glucose, 10g/1 (NH4)2SO4, 1.0g/l MgSO4, 1.0 g/l CaCl and assorted trace elements. Problem 3 (35 points) (a) The parent culture for a bacterial fermentation was grown at 30C in a medium containing 60 g/l glucose, 20 g/1 protein hydrolysate and 20g/l yeast extract Samples were inoculated into four subcultures as follows: 2 Medium A as for parent culture 106 Subculture B as for parent as for parent culture culture 10% 104 D see below Inoculum size (cells/ml) 10% Inoculum age 6 24 24 24 (h) The medium for subculture D contained: 60g/l glucose, 10g/1 (NH4)2SO4, 1.0g/l MgSO4, 1.0 g/l CaCl and assorted trace elements