Question 12-13

12. WHMIS Safety Data Sheets (SDS) rarely provides information on protein or enzymes. That's not to say they are not dangerous. For example, protein ricin is extremely toxic, and it has been used as a biochemical weapon in the past. a. What would happen to a person if a large amount of DNase I was accidentally ingested? (1pt) b. Based on the DNase I catalytic mechanism, if you have to mutate one amino acid residue to inactivate the enzyme, which residue would you mutate and why? (Ipt) 13. Would the following treatment display hyperchromic effect? Explain: a. After DNase I treatment at 37C, instead of slowly cooling to room temperature, you quickly cooled the diluted DNA sample on ice. (2pts) b. After boiling your diluted DNA sample, you slowly cooled it to room temperature instead of quickly cooling on ice. (2pts) c. You treated your diluted DNA sample with RNase L at 37C for 20min and allowed the sample to slowly cool to room temperature. (2pts) d. Given the pKa values of nucleobases below, you titrated your diluted DNA sample to pH=2. (2pts) strnnn