Question: You have a stock solution containing 1.25 mM of substrate in borate buffer pH 9.5. You transfer 1.25 mL of this stock into a

You have a stock solution containing 1.25 mM of substrate in borate

You have a stock solution containing 1.25 mM of substrate in borate buffer pH 9.5. You transfer 1.25 mL of this stock into a cuvette and then add 250 ul of enzyme solution to initiate the reaction. What will be the final concentration of substrate in the cuvette following addition of the enzyme? O 1042 mM O 1.25 mM O 0.00625 mM O 1.04 mM O 3750 mM

Step by Step Solution

★★★★★

3.50 Rating (153 Votes )

There are 3 Steps involved in it

1 Expert Approved Answer

Step: 1 Unlock

Ans 104 mM Explanation Taking125 ml of the stock so... View full answer

Question Has Been Solved by an Expert!

Get step-by-step solutions from verified subject matter experts

Step: 2 Unlock

Step: 3 Unlock

Document Format (2 attachments)

60d02c454241a_219570.pdf

180 KBs PDF File

60d02c454241a_219570.docx

120 KBs Word File

Students Have Also Explored These Related Chemistry Questions!

You have a stock solution certified by a manufacturer to contain 150.0 z 0.3 mg SO42- /mL. You would like to dilute it by a factor of 100 to obtain 1.500 mg SO42- /mL. Two possible dilution methods...

(a) You have a stock solution of 14.8 MNH3.How many milliliters of this solution should you dilute to make 1000.0 mL of 0.250 M NH3? (b) If you take a 10.0-mL portion of the stock solution and dilute...

You have a stock portfolio consisting of approximately 100 stockswhere each stock constitutes roughly 1% of the portfolio's total value. The firms constitute a variety of industries and economic...

The color of the wire that is connected between a switch and a lamp in domestic installation is a.blue b.brown c. purple d. yellow

solve for table 3.2 with the given data. you do not need to solve from assay 1 to 6, but at least show how you calculate one example of each column (corrected A410, p-nitrophenolate, p-nitrophenol...

Hello i need help with the calculations and the drawing of the graphs. Basically the last picture where says laboratory report, the part of results. KINETICS OF A SINGLE SUBSTRATE ENZYME-CATALYSED...

please answer all questions. Procedure is Experiment: Chymotrypsin Catalysis Prelab Assignment 1. 12 points) The table provided in Step 1 of Day 1's procedure outlines four different experimental...

of 1000 Hepl Protein Seguences. together, and then placed in a 80C freezer for 20min to cellulose, which is eesential because Hept binds sugarfreene the solation, followed by lyophilization to...

3.If you were to make a 12 mL extraction buffer, what volume of PMSF would you need to add? Why is PMSF added? 4.Calculate the volume of L-DOPA to add to get each of the concentrations used in the...

Temperature 4 C 21 C 30 C 37 C 90 C Absorbance at zero min Absorbance after 10 min of incubation A 0min A 10min Enzyme activity in 10 min (moles) Enzyme activity (moles pyruvate/min) CALCULATIONS:...

A couple who is very much in love decides to get married in 5 years; for which they need $ 12,000, both for the party and for their honeymoon. Both decide that each deposit $ 1,500 in a single bank,...

Help Desk Layout Using the internet, research help desk layouts. What types of novel layouts (i.e., not standard cubicles)? Which of these layouts would you prefer to work in?

ABN Amro Bank, Amsterdam, wants to purchase Rs.15 m against $ for funding their Vostro account with Canara Bank, New Delhi. Assuming the inter bank, rates of $ is 51.3625 - 3700, what would be the...

Operating expenses would include O interest expense. O income tax expense. O freight-out. O freight-out and interest.

Question 9 12 pts Each year about 1500 students take the Introductory statistics course at a large university. This year scores on the final exam are distributed with a median of 74 points, a mean of...

Solve 2 cos(x) - 3 cos(x) + 1 = 0 for all solutions 0

a. Prepare a vertical analysis of Comea Company's income statements. Round your answers to one decimal place. Cornea Company Income Statements For the Years Ended December 31 b. The vertical analysis...

6. (Potential Energy and Conservation of Energy) What should be the spring constant k of a spring designed to bring a 1200-kg car to rest from a speed of 95 km/h so that the occupants undergo a...

Write complete balanced half-reactions for (a) oxidation of nitrous acid to nitrate ion in acidic solution, (b) oxidation of N 2 to N 2 O in acidic solution.

The precipitation reaction between AgNO3(aq) and NaCl(aq) proceeds as follows: (a) By using Appendix C, calculate ÎH° for the net ionic equation of this reaction. (b) What would you expect...

Gold is alloyed (mixed) with other metals to increase its hardness in making jewelry. (a) Consider a piece of gold jewelry that weighs 9.85 g and has a volume of 0.675 cm3. The jewelry contains only...

In each case, identify any divergence between social and private costs. a. Cigarette smoking b. Getting a university education c. Private ownership of guns d. Drilling for offshore oil

Fill in the blanks to make the following statements correct. a. The "formal defence" of free markets is that, if all markets were perfectly competitive, then prices would equal ________ for all...

This question is based on Externalities and the Coase Theorem on the book's MyEconLab at www.myecon-lab.com. Consider a steel producer that dumps one litre of toxic waste into a river for every tonne...