The enzyme Fumarase has the following kinetic constants S E harr ES rarr P E where k1 and k 1 are the rate constants of the reversible reaction step (forward and reverse reaction, respectively) and k2is for the irreversible step The values of k1,k 1 ,and k2 are 10 9 M 1 s 1 , 4 4 x 10 4 s 1 , and 10 3 s 1 (a) What is the value of the Michaelis constant for fumarase (b) At an enzyme concentration of 10 6 M, what will be the initial rate of product formation at an initial substrate concentration of10 3 M

Question: The enzyme Fumarase has the following kinetic constants: S + E ↔ ES → P + E where k1 and k-1 are the rate constants

The enzyme Fumarase has the following kinetic constants: S + E ↔ ES → P + E where k1 and k-1 are the rate constants of the reversible reaction step (forward and reverse reaction, respectively) and k2is for the irreversible step. The values of k1,k ^_-1,and k2 are 10 ⁹ M ^-1 s ^-1, 4.4 x 10 ⁴ s ^-1, and 10 ³ s ^-1.

(a) What is the value of the Michaelis constant for fumarase?

(b) At an enzyme concentration of 10 ^-6 M, what will be the initial rate of product formation at an initial substrate concentration of10 ^-3 M?

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