Question: Three whey proteins ( A , B and C ) are being fractionated by isocratic chromatography using a 1 5 c m long cation exchange
Three whey proteins A B and C are being fractionated by isocratic chromatography using a long cation exchange column having theoretical plates. A mobile phase flow rate of resulted in retention times of and minutes respectively for proteins and C
a Calculate resolution and comment on the feasibility of separation for all three proteins.
b What the length of the column should be to achieve resolution of between peaks of proteins & B Assume that the elution volumes and the HETP value would remain the same.
Step by Step Solution
There are 3 Steps involved in it
1 Expert Approved Answer
Step: 1 Unlock
Question Has Been Solved by an Expert!
Get step-by-step solutions from verified subject matter experts
Step: 2 Unlock
Step: 3 Unlock
