Question: Three whey proteins ( A , B and C ) are being fractionated by isocratic chromatography using a 1 5 c m long cation exchange

Three whey proteins (A, B and C) are being fractionated by isocratic chromatography using a 15cm long cation exchange column having 90 theoretical plates. A mobile phase flow rate of 1.5mlmin-1 resulted in retention times of 10,18 and 23 minutes respectively for proteins A,B and C.
a) Calculate resolution and comment on the feasibility of separation for all three proteins.
10%
b) What the length of the column should be to achieve resolution of 1.5 between peaks of proteins A & B? Assume that the elution volumes and the HETP value would remain the same.
 Three whey proteins (A, B and C) are being fractionated by

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