Question: A bottle contains a mix of three amino acids in citrate buffer (pH 3.5). The amino acids in solution are Lysine, Valine, and Aspartate.

A bottle contains a mix of three amino acids in citrate buffer

A bottle contains a mix of three amino acids in citrate buffer (pH 3.5). The amino acids in solution are Lysine, Valine, and Aspartate. If a cation exchange column was set up and equilibrated with citrate buffer (pH 3.5) state which amino acid(s) would bind to the column and which amino acid(s), if any, woul through the column. Explain your reasoning. Word limit: 150 words. Note that the Isoelectric point (pl) at 25 C of the amino acids are: Lysine (pl 9.47) Valine (pl 6.02) Aspartate (pl 2.98)

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