a) Discuss your UV-VisBeer-Lambert plot. Does your calibration curve for fluorescein obey the Beer-Lambert Law? Why or why not? Were your standards accurately prepared? How does your R2 value support this conclusion?

b) Discuss your Fluorimetry Emission plot. Does your calibration curve for fluorescein obey a linear relationship? Why or why not? Were your standards accurately prepared? How does your R2 value support this conclusion?

c) Compare your percent recovery results for each method-are they the same or do they differ? If different, how much?

d) Which detection method (and/or instrument) is more sensitive? Why?

e) Which detection method (and/or instrument) is more selective? Why?

f) Which detection method (and/or instrument) has lowest detection limit? Why?

g) Which detection method (and/or instrument) has the largest linear dynamic range? Why?

h) Discuss the agreement (or disagreement) between the two methods and your confidence in the analysis. Discuss in detail at least 2 reasons why you think the results are in agreement or disagreement.

i) Discuss in detail 2 significant sources of experimental error for each method in this experiment. Justify how your sources of error would affect your results.

j) Given all of the data derived from this lab, which instrument would you recommend to a client for the continued high-throughput analysis of the fluorescein unknown provided. Give 2 reasons in detail why you would recommend the method you are selection and 2 reasons in detail why you would not recommend the other methods and instrument. When discussing your reasons, keep in mind all aspects of the experiment (i.e. time to prepare and analyze standards and samples, instrument setup, how long it would take to train staff on instrumental use, LOD/LOQ, selectivity, sensitivity etc.)