Two-dimensional gel electrophoresis of proteins in a cell extract provides a qualitative way to compare proteins with

Question:

Two-dimensional gel electrophoresis of proteins in a cell extract provides a qualitative way to compare proteins with respect to intracellular abundance. Describe a quantitative approach to determine the number of molecules of an enzyme per cell.

Fantastic news! We've Found the answer you've been seeking!

Step by Step Answer:

Related Book For book-img-for-question

Biochemistry Concepts and Connections

ISBN: 978-0321839923

1st edition

Authors: Dean R. Appling, Spencer J. Anthony Cahill, Christopher K. Mathews

See More Books

Question Posted: Apr 21, 2016 04:17 AM

See More Questions

Proteomics involves the global analysis of protein expression. In one approach, all the proteins in control cells and treated cells are extracted and subsequently separated using two-dimensional gel...
When proteins are separated using native gel electrophoresis, size, shape, and charge control their rate of migration on the gel. Why does DNA separate based on size, and why do we not worry much...
Te gel electrophoresis pattern in Figure 4.23 was determined by soaking the gel in a solution of ethidium bromide (EtBr). This is a uorescent molecule with a planar structure: Te at molecule...
8) Implement a class UserNames which requires an ArrayList of names (user names), consider the following static methods methods: initilizeUserName(), deleteUserName(), addUserName() and display...
On January 1, 2010, the Field Company acquired 40% of the North Company by purchasing 8,000 shares for $144,000 and obtained significant influence. On the date of acquisition, Field calculated that...
Suppose the MPC is .90 and the MPI is .10. If government expenditures go up $$100$ billion while taxes fall $$10$ billion, what happens to the equilibrium level of real GDP?
In a first system of pulleys, there are 4 movable pulleys. If the efficiency of the system is 80%, find the effort required to lift a load of 5 kN.
Joshua Company has determined the following selling price and manufacturing cost per unit based on normal production of 72,000 units per year: Selling price per...
You are a middle manager for a retail company. There are 8 employees who report to you, two full-time and six part-time. Employees work various shift schedules including day, evening, and weekend...
For each of the following, find the P-value using Table C. Then calculate a more precise value using technology. a. 2 = 19.03, df = 11 b. 2 = 19.03, df = 3 Table C Table C Chi-square distribution...
Wheeler and Mathews (J.Biol.Chem.286:16992-16996,2011) reported the concentrations of adenine nucleotides in rat liver mitochondria as follows: ATP, 5.5 mM; ADP, 5.1 mM; AMP, 1.8 mM. (a) Calculate...
Intracellular concentrations in resting muscle are as follows: fructose-6-phosphate, 1.0 mM; fructose-1,6-bisphosphate, 10 mM; AMP, 0.1 mM; ADP, 0.5 mM; ATP, 5 mM; and Pi , 10 mM. Is the...
Four analysts cover the stock of Fluorine Chemical. One forecasts a 5% return for the coming year. A second expects the return to be negative 5%. A third predicts a 10% return. A fourth expects a 3%...
1. If it takes 50 J of energy to move 10 C of charge from point A to point B. what is the magnitude of the potential difference between points A and B? 2. Two long parallel wires carry currents of 20...
ELMI Corporation acquired a barn and three acres of land for a lump-sum price of $2,400,000. The barn contained used but fully-functional farming equipment. According to independent appraisals, the...
Ashleigh Batista is 42 years old and divorced. Her net income for 2023 is $84,400. She has retained the family home and custody of both children, who live with her. Her son is 23 years old, has no...
One of the 5 major tax issues associated with mergers, acquisitions and divestitures is whether the acquirer uses cash from newly issued equity or cash from retained earnings to purchase the target...
A taxpayer purchased used business equipment on November 20, 2016, for $100,000. The equipment was sold for $60,000 on August 25, 2018. Depreciation information is as follows: Accelerated...
City RecSports Club (CRC) is run by the local government to offer tennis, basketball, and other athletic court facilities to the public. CRC has four locations in the metropolitan area, and each has...
During the month, services performed for customers on account amounted to $7,500 and collections from customers in payment of their accounts totaled $6,000. At the end of the month, the Accounts...
Consider the following peptides: Phe! Glu! Ser! Met and Val! Trp! Cys! Leu. Do these peptides have differ-ent net charges at pH 1? At pH 7? Indicate the charges at both pH values.
Can a peptide act as a buffer? If so, why?
Consider the peptides Ser! Glu! Gly! His! Ala and Gly! His! Ala! Glu! Ser. How do these two pep-tides differ?
The market price of a semi-annual pay bond is $957.95. It has 15.00 years to maturity and a coupon rate of 7.00%. Par value is $1,000. What is the yield to maturity? The market price of a semi-annual...
Compute the accumulated sum of the following payments: (a) $400 every six months for 4 years at 8% compounded semi-annually (b) $250 per month for 5 years at 7.5% compounded monthly.
The output is wrong. Sometimes a program lacking input will produce wrong output (as in this case), or no output. Remember to always pre-enter needed input. Type 2 in the input box, then click "Run...

Two-dimensional gel electrophoresis of proteins in a cell extract provides a qualitative way to compare proteins with

Question:

Step by Step Answer:

The most direct way is to purify the enzyme to homogenei...View the full answer

Biochemistry Concepts and Connections

Students also viewed these Medical Sciences questions