Question: Hydrazine cleaves amide bonds to form acylhydrazides according to the general mechanism of nucleophilic acyl substitution discussed in Chapter 20: This reaction forms the basis

Hydrazine cleaves amide to form acylhydrazides according to the general mechanism of nucleophilic acyl substitution discussed in Chapter 20:
This reaction forms the basis of one method of terminal residue analysis. A peptide is treated with excess hydrazine in order to cleave all the peptide linkages. One of the terminal amino acids is cleaved as the free amino acid and identified; all the other amino acid residues are converted to acylhydrazides. Which amino acid is identified by hydrazinolysis, the N terminus or the C terminus?

Step by Step Solution

★★★★★

3.41 Rating (160 Votes )

There are 3 Steps involved in it

1 Expert Approved Answer

Step: 1 Unlock

Consider for example the reaction of hydrazine with a very simple dipeptide su... View full answer

Question Has Been Solved by an Expert!

Get step-by-step solutions from verified subject matter experts

Step: 2 Unlock

Step: 3 Unlock

Document Format (1 attachment)

C-O-AA (32).docx

120 KBs Word File

Students Have Also Explored These Related Organic Chemistry Questions!

Hydrazine cleaves amide bonds to form acylhydrazides according to the general mechanism of nucleophilic acyl substitution discussed in Chapter 20: This reaction forms the basis of one method of...

When 3,3-dichloropentane is treated with excess sodium amide in liquid ammonia, the initial product is 2-pentyne: However, under these conditions, this internal alkyne quickly isomerizes to form a...

When 1, 2-dibromo-3, 5-dinitrobenzene is treated with excess NaOH at 50 C, only one of the bromine atoms is replaced. Draw an equation for this reaction, showing the product you expect. Give a...

It is becoming widely expected that interest rates will rise in the coming year. In recent weeks the 10 year US bond has risen from about 1.50% to 1.80%. Using the appendix for chapter 2 on Webster...

Kindly help to solve the questions with the use of the programmer, UCSF Chimera: Exploring protein structures in Chimera: This exercise walks through some of the features of Chimera that aid the...

With reference to Exercise 11.7 construct (a) A 95% confidence interval for the average weekly sales when $45 is spent on advertising; (b) A 95% prediction interval for the weekly sales when 845 is...

Could a third variable cause both the independent (predictor) and dependent (criterion) variables?

Firms such as Wal-Mart, General Electric, Chase Manhattan, and Boeing have a lot of influence in their respective supply chains because of the power they have. Explain how firms with a lot of power...

Ranger Corporation has decided to invest in renewable energy sources to meet part of its energy needs for production. It is considering solar power versus wind power. After considering cost savings...

1. How will you characterize Tupperwares distribution strategy in relation to the theoretical models? 2. What are the advantages and disadvantages of Tupperwares distribution model? 3. How do you...

On January 1, 2011, Encino Company issued bonds with a face value of $1,000,000 and a maturity date of December 31, 2020. The bonds have a stated interest rate of 8%, payable on January 1 and July 1....

Suppose the following high temperatures were recorded for major cities in the contiguous United States for a day in July. Construct and interpret a stem-and-leafdiagram. 84 92 96 91 96 94 93 82 81 76...

ue son Bonos Soberanos en el mercado financiero de chile (incluir referencia de la fuente)

KMC Airlines was established 40 years ago and currently operates a small fleet of planes that fly to various cities across Europe from their base in the East Midlands. Whilst they are not a market...

5-3 In a typical loan amortization schedule: the amount of interest paid each period does not remain constant. the amount of each payment does not remain constant. the amount of interest paid each...

R-STUDIO Please download real GDP (GDP (constant 2010 US$) ) and population between 1970-2010 from https://data.worldbank.org/ for the US, Europe Union, Japan, and China. 1. Please calculate real GDP...

How do you compute longhand Approximationg Binomial Probabilities In Exercises 19-26, determine whether you can use a normal distribution to approximate the binomial distribution. If you can, use the...