Let's suppose a DNA mutation changes the consensus sequence at the -35 location in a way that

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Let's suppose a DNA mutation changes the consensus sequence at the -35 location in a way that inhibits σ factor binding. Explain how a mutation could inhibit the binding of σ factor to the DNA. Look at Figure 12.5 and describe two specific base substitutions you think would inhibit the binding of σ factor. Explain why your base substitutions would have this effect.
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