1. A CSTR with a volume of 30 L, is fed at a flow rate of 1 L/min and the substrate concentration in the feed is 50 g/L. The reactor is used to perform an enzymatic reaction which follows Michaelis Menten kinetics. Initial experiments show that an enzyme concentration of 10 g/L in solution gives KM = 10 g/L and Vm = 6 g/L/min. The enzyme is covalently bound to a surface. In the reactor, kL = 2.5 · 10-3 m/min. a) If your measurements show that each square meter of surface bound 7 g of enzyme, calculate Vm'. State assumptions.

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