Question: Please help, and please don't copy paste from internet. 1. Why should you use the absorbance of an unknown sample that falls within the standard
Please help, and please don't copy paste from internet.
1. Why should you use the absorbance of an unknown sample that falls within the standard curve (ie. no extrapolation)?
2. Why is bovine serum albumin (BSA) used as the protein standard?
3. Explain the difficulties that you will encounter in determining the concentration of your sample if the absorbance is too high or too low.
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