Standard Reduction Potentials The standard reduction potential, E'', of any redox couple is defined for the half- cell reaction: Oxidant + n electrons Reducer The E' values for the NAD+/NADH and acetoacetate/B-hydroxybutyrate conjugate redox couples are -0.32 V and -0.35 V, respectively. 1- Which of the NAD+/NADH or the acetoacetate/B-hydroxybutyrate redox couple contains the strongest reducer (i.e. the form the most susceptible to donate its electrons)? which couple contains contains the strongest oxidant (i.e. the form the most susceptible to accept electrons)? Explain. 2- Starting using 1 M concentrations of each reactant and product at pH 7 and 25 C (i.e. biochemical standard conditions), in which direction will proceed the following reaction? Explain (Hint: Calculate the AE' of the reaction and comment its value to justify your response. Show your work). B-hydroxybutyrate + NAD+ A acetoacetate + NADH + H+ 3- Calculate the AG' associated with the conversion of B-hydroxybutyrate into acetoacetate (Hints: 2 electrons are transferred during this reaction and the Faraday constant, F = 96.5 kJ/V.mol). Show your work. 4- Calculate the equilibrium constant (K'eq) associated with the conversion of of B-hydroxybutyrate into acetoacetate. Use R=8.315 J/mol.K and show your work. Standard Reduction Potentials The standard reduction potential, E'', of any redox couple is defined for the half- cell reaction: Oxidant + n electrons Reducer The E' values for the NAD+/NADH and acetoacetate/B-hydroxybutyrate conjugate redox couples are -0.32 V and -0.35 V, respectively. 1- Which of the NAD+/NADH or the acetoacetate/B-hydroxybutyrate redox couple contains the strongest reducer (i.e. the form the most susceptible to donate its electrons)? which couple contains contains the strongest oxidant (i.e. the form the most susceptible to accept electrons)? Explain. 2- Starting using 1 M concentrations of each reactant and product at pH 7 and 25 C (i.e. biochemical standard conditions), in which direction will proceed the following reaction? Explain (Hint: Calculate the AE' of the reaction and comment its value to justify your response. Show your work). B-hydroxybutyrate + NAD+ A acetoacetate + NADH + H+ 3- Calculate the AG' associated with the conversion of B-hydroxybutyrate into acetoacetate (Hints: 2 electrons are transferred during this reaction and the Faraday constant, F = 96.5 kJ/V.mol). Show your work. 4- Calculate the equilibrium constant (K'eq) associated with the conversion of of B-hydroxybutyrate into acetoacetate. Use R=8.315 J/mol.K and show your work