Question: You want to make a 1M- succinate phosphate buffer that is pH 7 from two given stock solutions. Each is a 1M succinate phosphate buffer,
You want to make a 1M- succinate phosphate buffer that is pH 7 from two given stock solutions. Each is a 1M succinate phosphate buffer, but one is at a pH of 2 and the other is a pH of 12. Starting with pH2 and pH12 stock solutions how would you make 5mls of each pH between the two solutions.
| pH | Ratio of 2:12 | pH 2 volume | pH 12 volume |
| 2 | 10:0 | 5mls of pH 2 | 0mls of pH 12 |
| 3 | 9:1 | ||
| 4 | 8:2 | ||
| 5 | 7:3 | ||
| 6 | 6:4 | ||
| 7 | 5:5 | 2.5mls of pH 2 | 2.5mls of pH 12 |
| 8 | 4:6 | ||
| 9 | 3:7 | ||
| 10 | 2:8 | ||
| 11 | 1:9 | ||
| 12 | 0:10 | 0 ml of pH 2 | 5 ml of pH 12 |
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