Question: You want to make a 1M- succinate phosphate buffer that is pH 7 from two given stock solutions. Each is a 1M succinate phosphate buffer,

You want to make a 1M- succinate phosphate buffer that is pH 7 from two given stock solutions. Each is a 1M succinate phosphate buffer, but one is at a pH of 2 and the other is a pH of 12. Starting with pH2 and pH12 stock solutions how would you make 5mls of each pH between the two solutions.

pH Ratio of 2:12 pH 2 volume pH 12 volume
2 10:0 5mls of pH 2 0mls of pH 12
3 9:1

4

8:2
5 7:3
6 6:4
7 5:5 2.5mls of pH 2 2.5mls of pH 12
8 4:6
9 3:7
10 2:8
11 1:9
12 0:10 0 ml of pH 2 5 ml of pH 12

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